Can the biochemical incubator be used for plant tissue culture? How is it different from the light box?
Both the biochemical incubator and the light incubator are common box equipment in the laboratory. What is the difference between the two? The light incubator is mainly used to simulate the light conditions of plants, which is more suitable for experiments such as plant cultivation and seed germination, while the biochemical incubator focuses more on temperature and humidity control. Microbiological experiments are more suitable and are widely used in low temperature and constant temperature experiments. , Cultivation test, environmental test, etc.
There are many knowledge points that we need to master during the use of the biochemical incubator, such as: How to distinguish the pollution caused by endophytic bacteria during the experiment?
The first thing we need to know is that endophytic bacteria refer to bacteria that live in the interstitial or intracellular spaces of various tissues and organs of healthy plants at a certain stage or all stages of their life history. They can be histologically or strictly superficial. The method of isolating and amplifying microbial DNA from sterilized plant tissues or directly producing and amplifying microbial DNA to prove its endogenousness. In plant tissue culture, since the endophytic bacteria inside the material cannot be removed by general surface disinfection methods, as the material is brought into the culture process, the pollution caused by it is called endophytic bacterial contamination. From the point of view of the pollution state, it occurs around the plant material, and generally extends from the inside of the culture medium to the outside, and it takes longer than contact pollution. Contact bacteria generally show up in 2-4 days. Endophytic bacteria It will be more than 4 days, and the type of bacteria is relatively single.
In addition, the biochemical incubator is equipped with electric heating wire heating and compressor cooling at the same time. Therefore, it can be adapted to a large range, and it can be maintained at a constant temperature throughout the year, so it is gradually popular. The use and maintenance of this incubator is similar to an electric heating incubator. Because it is equipped with a compressor, you must also observe the precautions for refrigerator maintenance, such as maintaining a stable voltage, not excessively tilting, and timely cleaning the dust on the radiator.
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